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Monoclonal Antibody to mouse CD25 (Clone PC61) FITC Conjugate |
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Cat.No
| IMG-5924C-0100 | | IMG-5924C-0500 |
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Description
 | | CD25 |
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Format
| | FITC |
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Unit
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Price(USD)
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MSDS
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| Catalog No: |
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| Contents: |
Mouse CD25 specific monoclonal antibody at 0.5 mg/ml in 10 mM Tris, 150 mM NaCl, containing 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rat IgG1, Lambda |
| Clone: |
PC61 |
| Purification: |
Protein G Chromatography |
| Species: |
Mouse |
| Host: |
Rat |
| Application: |
Flow (Cell Surface): 0.1-1 ug/1x10^6 cells |
| Storage: |
Store at 4°C, stable for 6 months. DO NOT FREEZE; MATERIAL IS LIGHT-SENSITIVE. |
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Background
CD25 is a 55KDa transmembrane glycoprotein consisting of a heavily glycosylated extracellular domain, a transmembrane region and a short cytoplasmic tail. It is expressed during early stages of thymocytes development and in activated CD4+ memory T-lymphocytes. CD25 heterodimerizes with IL2R-beta and IL-2R gamma and forms the tripartite high affinity IL-2 receptor complex. It increases the specificity and affinity of IL-2 receptor complex for IL-2 ligand and is necessary for receptor clustering required for augmentation of IL-2 signaling. Mutations in CD25 result in severe immunodeficiency. |
Antigen
IL-2-dependent cytolytic mouse T-cell clone B6.1 |
Application Notes
Mouse splenocytes were stimulated with 5 ug/ml ConA for three days prior to testing. |
Genebank Info (Protein)
NP_000408 |
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Flow analysis of CD4 and CD25 on mouse splenocytes using 1 ug/1x10^6 cells of CD4 and CD25 antibody. |
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Reference
1. Leonard et al. Nature 300: 267, 1982.
2. Leonard et al. Proc Natl Acad Sci USA 80: 6957, 1983.
3. Erber et al. Am J Clin Pathol 89: 645, 1988.
4. Sharfe et.al PNAS 94; 3168-3171 (April 1997)
5. Vamosi et.al PNAS 101 (30); 11082-11087 (July 27, 2004)
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for six months from the date of purchase.
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